Effect of Trehalose and Trehalose Transport on the Tolerance of Clostridium perfringens to Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant
Table 2
Comparison of the gene transcription in wild type C. perfringens ATCC 13124 and gatifloxacin resistant mutant by qRT-.
Locus
Gene
Primer
Fold (mutant/wild)
CPF_0541 upstream
treB upstream
ACAGAGTACATAGGCGGAAAAGA
−333.89
−828.5
−330
TTTCAACATCTGCTTTGCTTG
CPF_0541
treB
TTGGGACTTTGGATTTGCTC
−93.81
−227.5
−22.28
GCCTGCCACTATAGCTCCTG
CPF_0542
treC
GAGCTTTAAAGGGCGAAGAAA
−45.1
−44.50
−16.80
CCCAGTTTAAATCAGCCTGTG
CPF_0543
treR
GCCTTCAGAATCACAACTTATGG
−1.69
−1.79
−1.31
TTTCGCACTCTTTTCCTAAGC
CPF_0539
RNA polymerase sigma-70 factor
TGTTGGATGAAAGTACACCGA
2.29
3.63
2.99
AGCCCTCCTTTAAAACCTCA
CPF_0069
Transcription antiterminator
ATTCGGCAAGAACAACAGGA
−90.74
−711.9
−116.6
GCAACCTTAAAGGATTCTGGA
CPF_0042
plc
TGACACAGGGGAATCACAAA
−9.58
−14.20
CGCTATCAACGGCAGTAACA
CPF_0623
revR
AGTCCTAATAGTAGATGATGAGGA
−1.92
−1.73
−1.51
CTTATAAGCTTTAGAGCTTCAGT
CPF_0007
gyrA
TGCCAGAATAGTTGGGGAAG
1.08
1.27
TACCATGTCCGTCAACAAGC
CPF_1204
vrr
CAAAAAGGATTTTAACAAGTGCAA
−596.80
−133.1
TTGATATTAAAGCAAGTATGGGACT
CPF_1956
topA
ACCTTGCAACTGACCCTGAT
−2.38
−2.42
−2.07
CAGCACTCAATCCCCATTTT
CPF_1760
topB
AACTTGGGCTTTAGGCCATT
−1.61
GCTACGAGCTCCCCTTCTCT
CPF_1785
PTS sucrose-specific IIBC component
GCGGCCATATTTGGAGTAAA
−92.15
CCAGTTCCAGCCAATTTCAT
treB and treC are trehalose-specific genes. The transcription of several genes was considerably decreased in the mutant in comparison with the wild type ATCC 13124; the data presented represent those obtained in different experiments. The expression of topoisomerase genes (gyr and top) and response regulator gene (revR) was not markedly changed. Pfo, which was also downregulated, was reported previously [28].