Mycobacterium bovis BCG Surface Antigens Expressed under the Granuloma-Like Conditions as Potential Inducers of the Protective Immunity

Table 2

Primers used for construction of recombinant M. bovis BCG surface antigens in the pET6xHN_N vector of E. coli.

Genes	Primers
Mb1524	5′-TTTTTGTCGACCAAGGAGCCGTTGCT-3′; 5′-TTTTTGAATTCCTATTGAGTCAACCTGGGGGG-3′
Mb01_03198	5′-TTTTTGTCGACGACACAACTGTCGCT-3′; 5′-TTTTTGAATTCCTAAGCGTCGATCCC-3′
PhoU1	5′-TTTTTGTCGACCGGACGGTCTAT-3′; 5′-TTTTTTGAATTCCAGTAAGTGGAAATCTCGTCCT-3′
HBHA	5′-TTTTTGTCGACGCTGAAAACTCGAAC-3′; 5′-TTTTTGAATTCCTACTTCTGGGTGACCTTCTT-3′