Prevalence and Characterization of Streptococcus pyogenes Clinical Isolates from Different Hospitals and Clinics in Mansoura

Table 1

Different oligonucleotides used in this work to detect different S. pyogenes genes.

Gene name	Type	Nucleotide sequences (5′ to 3′)	Amplicon size (bp)	Tm (°C)	Ref.
spy1258	Fw	AAAGACCGCCTTAACCACCT	407	56	[19]
	Rv	TGGCAAGGTAAACTTCTAAAGCA
speA	Fw	AGGTAGACTTCAATTTGGCTTGT	576	59	[6]
	Rv	GGGTGACCCTGTTACTCAC
speC	Fw	GCCAATTTCGATTCTGCCG	405	59
	Rv	TGCAGGGTAAATTTTTCAACGAC
speG	Fw	TGGAAGTCAATTAGCTTATGCA	384	59
	Rv	GCGAACAACCTCAGAGGGCAAA
speH	Fw	TGAGATATAATTGTCGCTACTCAC	480	59
	Rv	CCTGAGCGGTTACTTTCGG
speI	Fw	TTCATAGACGGCGTTCAACAA	176	59
	Rv	TGAAATCTAGAGGAGCGGCCA
speJ	Fw	TCCTTGTACTAGATGAGGTTGCA	286	59
	Rv	GGTGGGGTTACACCATCAG
speK	Fw	CCTTGTGTGTGTATCGCTTG	568	59
	Rv	TTGCTGTCCCCCATCAAA
speL	Fw	CCTGAGCCGTGAAATTCCC	657	59
	Rv	ACACCAGAATTGTCGTTTGGT
speM	Fw	ATCGCTCATCAAACTTTTC	496	59
	Rv	CCTTGTGTGTGTATCGCTTG
ssa	Fw	AAGAATACTCGTTGTAGCATGT	678	59
	Rv	AATATTGCTCCAGGTGCGG
smeZ	Fw	TTTCTCGTCCTGTGTTTGG	246	59
	Rv	TTCCAATCAAATGGGACGGAGAAC