Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles

Table 4

The KDT32 and KGT32 sequence analysis using EzTaxon databases.

Isolate	Completeness (%)	First top hit taxon	First top hit strain^T	Accession number	Similarity (%)	Diff/total nt	Top hit taxonomy
KDT32	68	Streptomyces lavenduligriseus	NRRL ISP-5487^T	JOBD01000085	99.8	2/985	Bacteria^D; Actinobacteria^P; Actinobacteria^CStreptomycetales^O; Streptomycetaceae^F; Streptomyces^G
KGT32	65.5	Streptomyces albidoflavus	ATCC25422 & DSM40455^T	Z76676	99.79	2/847	Bacteria^D; Actinobacteria^P; Actinobacteria^C; Streptomycetales^O; Streptomycetaceae^F; Streptomyces^G
		Streptomyces koyangensis	VK-A60^T	AY079156	99.79	2/947
		Streptomyces hydrogenans	NBRC 13475^T	AB184868	99.79	2/947
		Streptomyces daghestanicus	NRRL B-5418^T	DQ442497	99.79	2/946
		Streptomyces violascens	ISP 5183^T	AY999737	99.79	2/945

“Completeness” is the ratio of the length of our sequence to the full-length sequence; “Top hit taxon” is the first closet species in the database; “First top hit strain^T″ is the first top closet strain type; “Accession” is the ID for the closet species in the database; “Similarity” is the sequence similarity between the closet species and our isolate; “Different/total nucleotide” is the number of mismatched base pair between the closet species and our isolate from the total number of compared sequences; “Top hit taxonomy” is the taxonomical hierarchy of our isolate-based 16S rRNA gene sequence from Domain (Bacteria) to genus (Streptomyces){D = domain, P = phylum, C = class, O = order, F = family, G = genus}