Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq

Figure 4

RT-PCR detection of representative E. coli clone ST131 allele by analysis of amplicon melt curves. (a, c) Amplification curves for T-SNP and A-SNP expressions depending on SYBR Green I RT-PCR, respectively. (b) Thymine “T” assay for pabB with primers ST131 (TF/TR) to identify T144 single-nucleotide polymorphism (SNP); melting temperature (Tm: 82.9°C) = ST131 E. coli. (d) Adenine “A” assay for pabB with primers ST131 (AF/AR) to identify A450 single nucleotide polymorphism (SNP); melting temperature (Tm: 80.9°C) = ST131 E. coli.

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