Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya

Table 1

RT-PCR targeted genes and primer sequences with their expected amplicon size.

Gene	Primer pair	Primer sequence (5′ ⟶ 3′)	Amplicon size (bp)
aflR	Forward	CCGTCAGACAGCCACTGGACACGG	300
	Reverse	TGACCCACCTCTTCCCCCACG
aflS	Forward	GAACGCTGATTGCCAATGCC	1256
	Reverse	CGGTCAGGATGTTACTAAGC
aflD	Forward	ACCGCTACGCCGGCACTCTCGGAC	400
	Reverse	GTTGGCCGCCAGCTCTGACACTC
aflP	Forward	GTGGACGGACCTAGTCCGACATCC	624
	Reverse	GTCGGCGCCACGCACTGGGTTGGG
aflQ	Forward	TTAAGGCAGCGGAATACAAG	599
	Reverse	GACGCCCAAAGCCGAACACAAA
tub1	Forward	GCTTTCTGGCAAACCATCTC	1198
	Reverse	GGTCGTTCATGTTGCTCTCA