Purification, Characterization, and Biocatalytic and Antibiofilm Activity of a Novel Dextranase from Talaromyces sp.

Figure 7

Purification of dextranase by Sephadex G-100 chromatography. (a) The protein concentrations of different tube fractions eluted from the Sephadex G-100 column. (b) Analysis of purified dextranase (tube fractions 19–22) by 12% SDS-PAGE. Lane M: protein molecular mass marker (Thermo Scientific Spectra Multicolor Broad Range Protein Ladder); Lane 1: crude dextranase produced after optimization of production conditions; Lane 2: partially purified dextranase obtained by precipitation with 70% ammonium sulfate saturated solution; Lane 3: purified dextranase obtained by collecting of fraction tubes (19–22) after gel filtration chromatography.

(a)

(b)