Antibiotic Resistance and Virulence Gene Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Healthy Edible Marine Fish

Table 2

Oligonucleotide sequences used for the detection of staphylococcal enterotoxins, pvl, and SCCmec types.

Gene target/specificity	Sequence (5′-3′)	Product size (bp)	Reference
Universal fw	F: TGTATGTATGGAGGTGTAAC	—	[38]
Sea	R: ATTAACCGAAGGTTCTGT	270
Seb	R: ATAGTGACGAGTTAGGTA	165
Sec	R: AAGTACATTTTGTAAGTTCC	69
Sed	R: TTCGGGAAAATCACCCTTAA	306
See	R: GCCAAAGCTGTCTGAG	213
Luk-PVL	F: ATCATTAGGTAAAATGTCTGGACATGATCCA	433	[39]
	R: GCATCAAGTGTATTGGATAGCAAAAGC
SCCmec I	F: GCTTTAAAGAGTGTCGTTACAGG	613	[40]
	R: GTTCTCTCATAGTATGACGTCC
SCCmec II	F: CGTTGAAGATGATGAAGCG	398
	R: CGAAATCAATGGTTAATGGACC
SCCmec III	F: CCATATTGTGTACGATGCG	280
	R: CCTTAGTTGTCGTAACAGATCG
SCCmec Iva	F: GCCTTATTCGAAGAAACCG	776
	R: CTACTCTTCTGAAAAGCGTCG
SCCmec IVb	F: TCTGGAATTACTTCAGCTGC	493
	R: AAACAATATTGCTCTCCCTC
SCCmec IVc	F: ACAATATTTGTATTATCGGAGAGC	200
	R: TTGGTATGAGGTATTGCTAAAG
SCCmec IVd	F: CTCAAAATACGGACCCCAATACA	881
	R: TGCTCCAGTAATTGCTAAAG
SCCmec V	F: GAACATTGTTACTTAAATGAGCG	325
	R: TGAAAGTTGTACCCTTGACACC