Coexistence of Virulence Factors and Efflux Pump Genes in Clinical Isolates of Pseudomonas aeruginosa: Analysis of Biofilm-Forming Strains from Iran

Table 1

Oligonucleotide sequences used in this study and thermal cycling conditions.

Gene	Sequence of primers	Thermal cycles	Product size (bp)	Ref
mexA	F: CTCGACCCGATCTACGTC	95°C/5 min; (95°C/1 min, 56°C/30sec, 72°C/45sec) X30; 72°C/5 min	503	[8]
	R: GTCTTCACCTCGACACCC
mexB	F: TGTCGAAGTTTTTCATTGAG	95°C/5 min; (95°C/1 min, 58°C/45sec, 72°C/45sec) X35; 72°C/5 min	280	[8]
	R: AAGGTCAC GGTGATGGT
oprM	F: GATCCCCGACTACCAGCGCCCCG	95°C/5 min; (95°C/1 min, 57°C/1 min, 72°C/45sec) X30; 72°C/5 min	247	[8]
	R: ATGCGGTACTGCGCCCGGAAGGC
oprD	F: ATCTACCGCACAAACGATGAG	95°C/5 min; (95°C/1 min, 59°C/45sec, 72°C/1 min) X30; 72°C/5 min	156	[8]
	R: GCCGAAGCCGATATAATCAAAC
tetA	F: AAGAATCCGCGCGTTCAATCG	95°C/7 min; (95°C/1 min, 55°C/1 min, 72°C/50sec) X35; 72°C/10 min	138	[15]
	R: GCCCGGCACCGGCATAAT
tetR	F: CCGAATGCGTATGATTCTCC	95°C/10 min; (95°C/1 min, 57°C/30sec, 72°C/1 min) X30; 72°C/10 min	84	[15]
	R: CGCTTTACTGGCACTTCAGC
emrE	F: CCGCCATGACCAACTATCTC	95°C/5 min; (95°C/1 min, 58°C/1 min, 72°C/1 min) X30; 72°C/5 min	249	[6]
	R: GCTGGCCGTAGACGAACATC