Comparison of Different Phenotypic Tests versus PCR in the Detection of Carbapenemase-Producing Pseudomonas aeruginosa Isolates in Hamadan, Iran

Table 1

PCR primers for the detection of carbapenemase genes.

Carbapenemase genes	Sequence (5′–3′)	Primer	Expected amplicon size (bp)	Reference
IMP	Imp-F	GGA ATA GAG TGG CTT AAY TCT C	188	[18]
	Imp-R	CCA AAC YAC TAS GTT ATC T
VIM	Vim-F	GAT GGT GTT TGG TCG CAT A	390	[18]
	Vim-R	CGA ATG CGC AGC ACC AG
GIM-1	GIM-1F	TCG ACA CAC CTT GGT CTG AA	271	[18]
	GIM-2R	AAC TTC CAA CTT TGC CAT GC
SPM-1	SPM-1F	AAA ATC TGG GTA CGC AAA CG	477	[18]
	SPM-1R	ACA TTA TCC GCT GGA ACA GG
SIM-1	Sim-1F	TAC AAG GGA TTC GGC ATC G	570	[18]
	Sim-1R	TAA TGG CCT GTT CCC ATG TG
KPC 1-5	KPC-1F	CATTCAAGGGCTTTCTTGCTGC	538	[19]
	KPC-1R	ACGACGGCATAGTCATTTGC
AMPC	AMPC-F	CGGCTCGGTGAGCAAGACCTTC	218	[20]
	AMPC-R	AGTCGCGGATCTGTGCCTGGTC
OXA-48	OXA-48-F	GCTTGATCGCCCTCGATT	281	[21]
	OXA-48-R	GATTTGCTCCGTGGCCGAAA