Isolation and Characterization of Potential Antibiotic-Producing Actinomycetes from Water and Soil Sediments of Different Regions of Nepal
Table 1
Identification of actinomycetal isolates based on morphological and cultural characteristics.
Colony characteristics on SCA
Microscopic characteristics
Actinomycetes isolated
Light yellow-orange to orange-red colonies, occasionally brown maroon or blue green, the dark brown to black spore colonies’ surface darkens with spores
Fine substrate mycelium with spores like a cluster of grapes, no aerial mycelium
Micromonospora
The colony appears waxy and shiny; several millimeters in diameter; aerial filaments are formed; and the colony surface becomes dull and fuzzy
Gram positive, non-acid-fast, show pleomorphically ranging from a bacillary to coccoid structure; occasionally limited mycelium is found, which fragments readily to produce rod-shaped or coccoid cells
Nocardia
Powdery, colony appears convex, concave or flat surface; white gray to pinkish color colony
Filaments long highly branched and nonfragmented aerial filaments with spiral coil or multiple branching and long-chain spores
