Overexpression of mfpA Gene Increases Ciprofloxacin Resistance in Mycobacterium smegmatis

Table 2

Primers used in this study.

Primer name	Primer sequence
mfp5	TTTTTTGGTACCTACCGGTATTCGGCGCGAT
mfp11	TTTTTTGGTACCCGCCCAACCGTGCCATCG
mfp16	TTTTTTGGATCCGCTGCCCGGCTGAGGCTT
mfp17	TTTTTTATCGATCTGCTCGCGGTGAGAAAC
Random primers 9	(d(N)9 where N = A, C, G, T)
1	TTGCAGCAGCGGGTGGATTC
10	GACGAGGATCTGGAGCCGGCAT
11	GAGCGTGAAGTCGCACTCGA
2	AAACGACAACGGTGCCGAGG
3	GCGTTCGACCGGCATGTTCT
4	GGCTACGTGCTGCAATCGGT
5	GCGCGTTGGGTGTGTCGAGA
6	GCGTGGCGAGAGTGCTGATC
7	GTGGTGGTGCGTTTGTCGGG
8	GCCAAAGCATCCCACGCACG
9	CGTGCTGTGCCAGATGGTGC
16S forward	CCAGCAGCCGCGGTAATACG
16S reverse	ATCGG(C/T)TACCTTGTTACGACTTC

Underlined letters indicate restriction sites. Figure 1 shows target sites of primers in mfpEA operon.