Research Article
Antibiotics Resistance in Escherichia coli Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014–2019
Table 1
List of primers used in the study.
| | Antibiotic | Gene | DNA sequence (5’-3’) | Annealing temperature (°C) | Product (bp) | Reference |
| | Gentamicin | aph(6)-Id | F: ATCGTCAAGGGATTGAAACC
R: GGATCGTAGAACATATTGGC | 50 | 509 | [25] | | aph(3')-Ia | F: ATGGGCTCGCGATAATGTCG
R: AGAAAAACTCATCGAGCATC | 60 | 734 | [23] | | aac(3)-IV | F: CTTCAGGATGGCAAGTTGGT
R: TCATCTCGTTCTCCGCTCAT | 55 | 286 | [28] | | Ampicillin | blaSHV | F: TCGCCTGTGTATTATCTCCC
R: CGCAGATAAATCACCACAATG | 52 | 768 | | blaCMY | F: TGGCCAGAACTGACAGGCAAA
R: TTTCTCCTGAACGTGGCTGGC | 47 | 462 | | blaTEM-1B | F: TCCTTGAGAGTTTTCGCCCC
R: TGACTCCCCGTCGTGTAGAT | 60 | 634 | This study | | Sulfamethoxazole | sul1 | F: TTCGGCATTCTGAATCTCAC
R: ATGATCTAACCCTCGGTCTC | 47 | 822 | [28] | | Sul2 | F: CACATTGCGGCGTTCTTTGA
R: TTGCGGTTTCTTTTAGCGCC | 60 | 241 | This study | | Sul3 | F: AGTGGGCGTTGTGGAAGAAA
R: AGTAGCTGCACCAATTCGCT | 60 | 361 | | Trimethoprim | dfrA1 | F: GGAGTGCCAAAGGTGAACAGC
R: GAGGCGAAGTCTTGGGTAAAAAC | 45 | 367 | [27] | | dfrA17 | F: GGCGTAATCGGTAGTGGTCC
R: CCCCCGCCAGAGACATATAC | 60 | 263 | This study | | Fluoroquinolone | qnrA | F: GGGTATGGATATTATTGATAAAG
R: CTAATCCGGCAGCACTATTTA | 50 | 670 | [24] | | aac(6’)-Ib-cr | F: GCGATGCTCTATGAGTGGCT
R: TTTCTTCTTCCCACCGTCCG | 60 | 220 | This study | | Tetracycline | tet(A) | F: GGTTCACTCGAACGACGTCA
R: CTGTCCGACAAGTTGCATGA | 57 | 577 | [26] | | tet(B) | F: CCTCAGCTTCTCAACGCGTG
R: GCACCTTGCTGATGACTCTT | 56 | 634 |
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