Detection of blaOXA-145, blaOXA-224, blaOXA-539, and blaOXA-675 Genes and Carbapenem-Hydrolyzing Class D β-Lactamases (CHDLs) in Clinical Isolates of Pseudomonas aeruginosa Collected from West of Iran, Hamadan

Table 1

Oligonucleotide sequences used in this study and thermal cycling conditions.

Gene	Sequence of primers	Thermal cycles	Product size (bp)	Ref
blaOXA-145	F: CAAATGGGACGGAAAGCCAA; R: AGCTGACCCTCCCAGAATTT	95°C/5 min; (95°C/1 min, 60°C/30sec, 72°C/45 sec) X30; 72°C/5 min	204	This study
blaOXA-224	F: AGTGTGACGGAATCGTTGCT; R: GGCGCGGCTTAACTCAAGCGT	95°C/5 min; (95°C/1 min, 61°C/1 min, 72°C/45 sec) X30; 72°C/5 min	168	This study
blaOXA-539	F: TGATGCACTGGCGCTGCTGT; R: GATTTTTCGATGGGACGGCG	95°C/5 min; (95°C/1 min, 60°C/45 sec, 72°C/1 min) X30; 72°C/5 min	185	This study
blaOXA-675	F: TCAGCATCAAAAGAAAATCAGC; R: ATGATTTTGGTGGGAATGGA	95°C/7 min; (95°C/1 min, 60°C/1 min, 72°C/50 sec) X35; 72°C/10 min	138	This study