Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection

Figure 2

Fetuin-A content is reduced following intranasal Chlamydia challenge and is associated with increased IFN-γ content in the lung. Age-matched wild-type (WT) C57BL/6 and fetuin-A-deficient AHSG mice were challenged intranasally with 500 IFU Cm. Lung tissue (days 4 and 12) was collected and homogenized as described under “Materials and Methods.” (a) Fetuin-A content. (b) IFN-γ content. (c) Relative expression (fold-change) of TBX21, SOCS1, HMGB1, IL-4, SMS, and CCL3 genes. Determination of fetuin-A and IFN-γ content and real-time quantitative reverse transcription analysis were carried out as described under “Materials and Methods.” Respective bars represent the mean ± SD. Differences between indicated groups were analysed by one-way ANOVA with Tukey’s multiple comparison test. , , , and .

(a)

(b)

(c)