Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection

Figure 3

Regulatory role of fetuin-A in adaptive immunity following pulmonary Chlamydia infection. Age-matched wild-type (WT) C57BL/6 and fetuin-A-deficient AHSG mice were challenged intranasally with 500 IFU Cm. (a) Splenocyte IFN-γ secretion (n = 6 per group) from day 12 after Cm challenged animals stimulated with medium alone, UV-inactivated Cm, or anti-CD3 antibody. IFN-γ was determined 72 hours poststimulation. (b) Antichlamydial protease-like activity factor (CPAF) elicited titers. Serum (n = 6 per group) was collected at day 12 after Cm challenge. Preparation of splenocytes, IFN-γ quantitation by sandwich ELISA, collection of serum, and determination of CPAF titer were carried out as described under “Materials and Methods.” Respective bars represent the mean ± SD. Differences between indicated groups were analysed by one-way ANOVA with Tukey’s multiple comparison test. . ns, differences not statistically significant as determined by the two-tailed Student’ t-test.

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