Review Article

Current Ethanol Production Requirements for the Yeast Saccharomyces cerevisiae

Table 1

Different strategies used for the isolation of Saccharomyces cerevisiae from environmental sources.

OriginIsolation techniqueIsolated useReference

Grapes-spontaneously fermented musts15 to 20 grapes were placed in 150 mL of MEM malt extract medium and cultivated for 10 days at 23°CSearch for isolates with new oenological properties for wine production[106]

Spontaneously fermented mustsThe must fermentation process was carried out in a 1000 L barrel, at 18°CSearch for supply of isolates with new oenological properties for wine production[106]

Pineapple and orange peel1 gram of sample was soaked in 250 ml of YMM yeast maintenance medium at 30°C for 3 daysHigh-potential, stress-tolerant, ethanol-producing yeasts[107]

Cucumber jangajjiThree yeast strains were fortified from cucumber jangajji using a YM medium at 25°C for 48 hProbiotics and important crops for affected foods[108]

Bark of three tree species: Quercus rubra, unidentified tree. Tapirira guianensis2 g of each sample was inoculated into flasks with RE medium and, after turbidity, aliquots were seeded in YMA with 8% ethanolSecond-generation ethanol industrial processes[109]

MangoSpontaneous mango fermentation took place for 7 days, and every 24 hours the sample was diluted, seeded on GPY agar medium and incubated at 30°C for 3 daysCognac production[110]

Palm wineBeverage samples (1 mL) were directly diluted and plated in medium (YPD) and incubated at 30°C for 3–5 daysEvaluation of the genetic diversity and population structure of yeasts[111]

Distilleries in northeast BrazilMust samples were plated onto WLN medium containing nalidixic acid and ampicillin (both at 50 μg ml−1) after appropriate dilutionsEthanol production[112]

Distillery wastes, sewage and algal bloom and dairy wastesThe samples were mixed with YEPD broth and incubated at 40°C at 150 rpm. After 24 h, 100 μl of the diluted samples was spread on YEPD agar plates and incubated at 40°C for 48 h.Ethanol production[113]