Tetracycline-Resistant Genes in Escherichia coli from Clinical and Nonclinical Sources in Rivers State, Nigeria

Table 1

Primer sequences for the detection of E. coli tetracycline-resistant gene fragment detection.

Gene	Primer name	Primer sequence (5′ to 3′)	Annealing temp (°C)	Product size (bp)	References
tetA	tetA F; tetA R	TACATCCTGCTTGCCTT; AGATCGCCGTGAAGAG	62	205	[5]
tetB	tetB F; tetB R	CATTAATAGGCCCATCGCTG; TGAAGGTCATCGATAGCAGG	58	929	[7]
tetG	tetG F; tetG R	GCTCGGTGGTATCTCTGCTC; AGCAACAGAATCGGGAACAC	52	468	[1]
tetM	tetM F; tetM R	ACAGAAAGCTTATTATATAAC; TGGCGTGTCTATGATGTTCAC	55	171	[15]