Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso

Table 2

Primer sequences, PRC conditions, and the source of primers for amplification of antimicrobial resistance genes used for the study.

Target genes		Primer sequence (5′ to 3′)	PCR conditions	Product size	Reference
bla TEM	F	ACC​AAT​GCT​TAA​TCA​GTG​AG	3 min at 94°C; 35 cycles of 1 min at 94 C, 1 min. at 50°C and 1 min at 72°C; 10 min at 72°C	857 bp	[23]
	R	ACC​AAT​GCT​TAA​TCA​GTG​AG
temA	F	ATG​AGT​ATT​CAA​CAT​TTC​CG	3 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 62°C and 1 min at 72°C; 7 min at 72°C	867 bp	[23]
	R	CTG​ACA​GTT​ACC​AAT​GCT​TA
temB	F	TTT​TCG​TGT​CGC​CCT​TAT​TCC	3 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 62°C and 1 min at 72°C; 7 min at 72°C	798 bp	[23]
	R	CGT​TCA​TCC​ATA​GTT​GCC​TGA​CTC
tet(A)	F	GTG​AAA​CCC​AAC​ATA​CCC​C	5 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 60°C and 30 s at 72°C; 7 min at 72°C	888 bp	[24]
	R	GAA​GGC​AAG​CAG​GAT​GTA​G
tet(B)	F	CCT​TAT​CAT​GCC​AGT​CTT​GC	5 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 60°C and 30 s at 72°C; 7 min at 72°C	774 bp	[24]
	R	ACTGCCGTTTTTTCGCC
Sul1	F	TCA​CCG​AGG​ACT​CCT​TCT​TC	3 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 53°C and 1 min at 72°C; 7 min at 72°C	435 bp	[24]
	R	CAG​TCC​GCC​TCA​GCA​ATA​TC
Sul2	F	GCG​CTC​AAG​GCA​GAT​GGC​AT	3 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 53°C and 1 min at 72°C; 7 min at 72°C	293 bp	[24]
	R	GCG​TTT​GAT​ACC​GGC​ACC​CT
StrA	F	CCA​ATC​GCA​GAT​AGA​AGG​C	3 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 58°C and 1 min at 72°C; 7 min at 72°C	548 bp	[25]
	R	CTT​GGT​GAT​AAC​GGC​AAT​TC
aadA	F	GTG​GAT​GGC​GGC​CTG​AAG​CC	3 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 58°C and 1 min at 72°C; 7 min at 72°C	528 bp	[25]
	R	AATGCCCAGTCGGCAGCG
invA	F	TCA​TCG​CAC​CGT​CAA​AGG​AAC​C	30 s at 95°C; 34 cycles of 30 s at 95°C, 30 s at 58°C and 1 min at 72°C; 5 min at 72°C	284 bp	[26]
	R	GTG​AAA​TTA​TCG​CCA​CGT​TCG​GGC​AA
iroB	F	TGC​GTA​TTC​TGT​TTG​TCG​GTC​C	30 s at 95°C; 34 cycles of 30 s at 95°C, 40 s at 55°C and 1 min at 72°C; 5 min at 72°C	606 bp	[26]
	R	TAC​GTT​CCC​ACC​ATT​CTT​CCC
spiC	F	CCT​GGA​TAA​TGA​CTA​TTG​AT	12 min at 94°C; 34 cycles of 1 min at 94°C, 30 s at 54°C and 5 min at 72°C; 5 min à 72°C	309 bp	[26]
	R	AGT​TTA​TGG​TGA​TTG​CGT​AT
misL	F	GTC​GGC​GAA​TGC​CGC​GAA​TA	3 min at 94°C; 35 cycles of 1 min at 94°C, 1 min·s at 58°C and 1 min at 72°C; 5 min at 72°C	400 bp	[26]
	R	GCG​CTG​TTA​ACG​CTA​ATA​GT
orfL	F	GGA​GTA​TCG​ATA​AAG​ATG​TT	3 min at 94°C; 35 cycles of 1 min at 94°C, 1 min·s at 58°C and 1 min at 72°C; 5 min at 72°C	550 bp	[26]
	R	GCG​CGT​AAC​GTC​AGA​ATC​AA
pipD	F	CGG​CGA​TTC​ATG​ACT​TTG​AT	5 min at 94°C; 34 cycles of 25 s at 94°C, 30 s at 56°C and 50 s at 72°C; 5 min at 72°C	350 bp	[26]
	R	CGT​TAT​CAT​TCG​GAT​CGT​AA

5 s were added to the extension time each cycle.