Analysis of Amino Acid Changes in the Fusion Protein of Virulent Newcastle Disease Virus from Vaccinated Poultry in Nigerian Isolates
Table 7
Point mutation pattern along fusion protein of study isolates compared to reference LaSota strain KU665482.1.
ID virus isolate
Nucleotide at indicated position along the fusion gene
Hypervariable region/Signal peptide
AGR
Cleavage site
4
11
15
28
29
31
78
112
113
Codon
10–12
31–33
43–45
82–87
85–87
TCC
232–234
334–336
337–339
KU665482.1 LaSota.71.IR/2016†
AGA
GCA
CTG
CCG
GCA
CCC (S31P)
AAA
GGC
AGA
OK491971 Avian orthoavulavirus 1 isolate KN 14‡
AAA (P4K)
GTA (A11V)
CTG
CTG (L28P)
GCA
CCC (S31P)
AGA (K78R)
AGA (GII2R)
CGA (R113)
OK491972 Avian orthoavulavirus 1 isolate KN 36‡
AAA (P4K)
GTA (A11V)
CTG
CCG
ACA (A29T)
CCC (S31P)
AGA (K78R)
AGA (GII2R)
CGA (R113)
OK491973 Avian orthoavulavirus 1 isolate KN 48‡
AAA (P4K)
GTA (A11V)
CTG
CTG (L28P)
ACA (A29T)
CCC (S31P)
AGA (K78R)
AGA (GII2R)
CGA (R113)
OK491974 Avian orthoavulavirus 1 isolate KN 55‡
AAA (P4K)
GTA (A11V)
CTG
CTG (L28P)
ACA (A29T)
CCC (S31P)
AGA (K78R)
AGA (GII2R)
CGA (R113)
OK491975 avian orthoavulavirus 1 isolate KN 56‡
AAA (P4K)
GTA (A11V)
CTG
CTG (L28P)
GCA
CCC (S31P)
AGA (K78R)
AGA (GII2R)
CGA (R113)
OK491976 Avian orthoavulavirus 1 isolate KN 71‡
GAA (P4E)
GAA (A11E)
CAG (L15Q)
CTG (L28P)
ACA (A29T)
CCC (S31P)
AGA (K78R)
AGA (GII2R)
CGA (R113)
OK491977 Avian orthoavulavirus 1 isolate KN 75‡
AAA (P4K)
GTA (A11V)
CTG
CTG (L28P)
ACA (A29T)
CCC (S31P)
AGA (K78R)
AGA (GII2R)
CGA (R113)
Variable positions along functional sites in the fusion protein showing nucleotide substitution compared with LaSota KU665482.1 vaccine strain as reference. Not all substitution resulted in mutation because of degeneracy of amino acid. Italic positions show substitution site. Reference strain. †Vaccine strain. ‡Study isolates. AGR, antigenic region; A: adenine; G: guanine; C: cytosine; T: thymine.
