Analysis of Amino Acid Changes in the Fusion Protein of Virulent Newcastle Disease Virus from Vaccinated Poultry in Nigerian Isolates
Table 8
Point mutation pattern along fusion protein of study isolates compared to reference LaSota strain KU665482.1.
ID virus isolate
Nucleotide at indicated position along the fusion gene
Cleavage site
Fusion peptide
114
115
116
117
118
121
124
135
Codon
340–342
343–345
346–348
349–351
352–354
361–363
370–372
403–405
KU665482.1 LaSota.71.IR/2016†
CAG
GGC
CGC
CTT
ATA
ATT
GGT
ATA
OK491971 Avian orthoavulavirus 1 isolate KN 14‡
CGG (Q114R)
AAG (G115K)
CGT (R116)
TTT (L117F)
GTG (I118V)
GTT (I121V)
AGT (G124S)
GTA (I135V)
OK491972 Avian orthoavulavirus 1 isolate KN 36‡
CGG (Q114R)
AAG (G115K)
CGT (R116)
TTT (L117F)
GTG (I118V)
GTT (I121V)
AGT (G124S)
GTA (I135V)
OK491973 Avian orthoavulavirus 1 isolate KN 48‡
CGG (Q114R)
AAA (G115K)
CGT (R116)
TTT (L117F)
GTG (I118V)
GTT (I121V)
AGT (G124S)
GTA (I135V)
OK491974 Avian orthoavulavirus 1 isolate KN 55‡
CGG (Q114R)
AAG (G115K)
CGT (R116)
TTT (L117F)
GTG (I118V)
GTT (I121V)
AGT (G124S)
GTA (I135V)
OK491975 Avian orthoavulavirus 1 isolate KN 56‡
CGG (Q114R)
AAG (G115K)
CGT (R116)
TTT (L117F)
GTG (I118V)
GTT (I121V)
AGT (G124S)
GTA (I135V)
OK491976 Avian orthoavulavirus 1 isolate KN 71‡
CGG (Q114R)
AAA (G115K)
CGT (R116)
TTT (L117F)
GTA (I118V)
GTT (I121V)
AGT (G124S)
GTA (I135V)
OK491977 Avian orthoavulavirus 1 isolate KN 75‡
CGG (Q114R)
AAG (G115K)
CGT (R116)
TTT (L117F)
GTG (I118V)
GTT (I121V)
AGT (G124S)
GTA (I135V)
Variable positions along functional sites in the fusion protein showing nucleotide substitution compared with LaSota KU665482.1 vaccine strain as reference. Not all substitution resulted in mutation because of degeneracy of amino acid. Italic positions show substitution site. Reference strain. †Vaccine strain. ‡Study isolates. A: adenine; G: guanine; C: cytosine; T: thymine.
