Analysis of Amino Acid Changes in the Fusion Protein of Virulent Newcastle Disease Virus from Vaccinated Poultry in Nigerian Isolates
Table 9
Point mutation pattern along fusion protein of study isolates compared to reference LaSota strain KU665482.1.
ID virus isolate
Nucleotide at indicated position along the fusion gene
HRa
HRb
Conserved cysteine residue
145
272
278
285
288
297
394
Codon
433–435
814–816
832–834
862–864
862–864
889–891
1180–1182
KU665482.1 LaSota.71.IR/2016†
AAA
AAC
TCA
ATA
ACT
AAT
TGC
OK491971 Avian orthoavulavirus 1 isolate KN 14‡
AAC (K145N)
TAC (N272Y)
CCA (S278P)
ATA
AAA (T288N)
AAT
TGC
OK491972 Avian orthoavulavirus 1 isolate KN 36‡
AAC (K145N)
TAC (N272Y)
CCA (S278P)
ATA
AAA (T288N)
AAT
TGC
OK491973 Avian orthoavulavirus 1 isolate KN 48‡
AAC (K145N)
TAC (N272Y)
CCA (S278P)
AAA (I285K)
AAA (T288N)
AAA (N297K)
TGC
OK491974 Avian orthoavulavirus 1 isolate KN 55‡
AAC (K145N)
TAC (N272Y)
CCA (S278P)
ATA
AAA (T288N)
AAT
TGC
OK491975 Avian orthoavulavirus 1 isolate KN 56‡
AAC (K145N)
TAC (N272Y)
CCA (S278P)
AAA (I285K)
AAA (T288N)
AAT
TGC
OK491976 Avian orthoavulavirus 1 isolate KN 71‡
AAC (K145N)
TAC (N272Y)
CCA (S278P)
ATA
AAA (T288N)
AAT
TGC
OK491977 Avian orthoavulavirus 1 isolate KN 75‡
AAC (K145N)
TAC (N272Y)
CCA (S278P)
ATA
AAA (T288N)
AAT
TGT (C394S)
Variable positions along functional sites in the fusion protein showing nucleotide substitution compared with LaSota KU665482.1 vaccine strain as reference. Not all substitution resulted in mutation because of degeneracy nature of amino acid. Italic positions show substitution site. Reference strain. †Vaccine strain. ‡Study isolates. HR: heptad repeats; A: adenine; G: guanine; C: cytosine; T: thymine.
