Predominance of OXA-48 Carbapenemase-Producing Enterobacterales in a Moroccan Hospital

Table 1

Sequences of primers used for multiplex PCR.

PCR name	Targeted gene	Primer name	Sequence (5′ to 3′ end)	Amplicon size (bp)	Melting temperature (°C)	Reference
Multiplex 1	blaKPC type	Forward	TGT​TGC​TGA​AGG​AGT​TGG​GC	340	56	[15]
		Reverse	ACG​ACG​GCA​TAG​TCA​TTT​GC
	blaOXA-48-like including OXA-199 and OXA-370	Forward	AAC​GGG​CGA​ACC​AAG​CAT​TTT	597		[15]
		Reverse	TGA​GCA​CTT​CTT​TTG​TGA​TGG​CT
	RepA	Forward	GAC​ATT​GAG​TCA​GTA​GAA​GG	905		[16]
		Reverse	CGT​GCA​GTT​CGT​CTT​TCG​GC
	KPC	Forward	CTG​TCT​TGT​CTC​TCA​TGG​CC	796		[17]
		Reverse	CCT​CGC​TGT​GCT​TGT​CAT​CC
Multiplex 2	blaVIM type	Forward	CGCGGAGATTGARAAGCAAA	247	56	[15]
		Reverse	CGCAGCACCRGGATAGAARA
	VIM2	Forward	ATG​TTC​AAA​CTT​TTG​AGT​AAG	801		[18]
		Reverse	CTACTCAACGACTGAGCG
	NDM-1	Forward	CAT​TTG​CGG​GGT​TTT​TAA​TG	998		[19]
		Reverse	CTG​GGT​CGA​GGT​CAG​GAT​AG
Multiplex 3	blaNDM-1	Forward	TAA​AAT​ACC​TTG​AGC​GGG​C	439	52	[15]
		Reverse	AAATGGAAACTGGCGACC
	blaIMP variants except, IMP-3, IMP-16, IMP-27, IMP-31, IMP-34, and IMP-35	Forward	GAGTGGCTTAATTCTCRATC	183		[20]
		Reverse	CCAAACYACTASGTTATCT
	blaOXA-23-like	Forward	GTG​GTT​GCT​TCT​CTT​TTT​CT	736		[15]
		Reverse	ATT​TCT​GAC​CGC​ATT​TCC​AT
Multiplex 4	OXA-58-like	Forward	TGGCACGCATTTAGACCG	507	52	[21]
		Reverse	AAACCCACATACCAACCC
	VIM1	Forward	AGT​GGT​GAG​TAT​CCG​ACA​G	261		[20]
		Reverse	ATGAAAGTGCGTGGAGAC
	NDM	Forward	GGT​TTG​GCG​ATC​TGG​TTT​TC	621		[21]
		Reverse	CGG​AAT​GGC​TCA​TCA​CGA​TC
Multiplex 5	OXA-23-like	Forward	GAT​CGG​ATT​GGA​GAA​CCA​GA	501	52	[21]
		Reverse	ATT​TCT​GAC​CGC​ATT​TCC​AT
	OXA-24-like	Forward	TTC​CCC​TAA​CAT​GAA​TTT​GT	1024		[21]
		Reverse	GTA​CTA​ATC​AAA​GTT​GTG​AA
	OXA-51-like	Forward	TAA​TGC​TTT​GAT​CGG​CCT​TG	353		[21]
		Reverse	TGG​ATT​GCA​CTT​CAT​CTT​GG

For degenerate primers: R = A or G; S = G or C; Y = C or T.