Association of qnr Genes and OqxAB Efflux Pump in Fluoroquinolone-Resistant Klebsiella pneumoniae Strains

Table 1

PCR program for detection of oqxA and oqxB and qnr genes.

Primer	Initial denaturation	Denaturation	Annealing	Elongation	Final extension	Cycle
OqxA	94°C	94°C	56°C	72°C	72°C	25
	2 min	15 s	30 s	1 min	7 min
OqxB	94°C	94°C	55°C	72°C	72°C	32
	2 min	30 s	30 s	1 min	10 min
qnrA, B, S	94°C	94°C	57°C	72°C	72°C	30
	5 min	1 min	1 min	1 min	5 min