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International Journal of Microbiology
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International Journal of Microbiology
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2024
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Article
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Tab 1
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Research Article
Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples
Table 1
Cq (and standard deviation) obtained for different copy number of templates to evaluate the limit of detection (LoD).
Gene target
Copies per reaction
1
5
10
25
E
N
E
N
E
N
E
N
Replicas
18
18
18
18
18
18
18
18
Positive
7
12
11
18
18
18
18
18
Cq (mean)
40.64
39.79
39.35
36.83
36.63
35.54
34.60
33.25
SD
0.44
1.10
0.81
0.57
0.37
0.45
0.53
0.63