Involvement of the Bacillus SecYEG Pathway in Biosurfactant Production and Biofilm Formation

Table 1

Sequences of SecDF primers used in this work.

Primers	Sequences (5′……3′)	Size (pb)	Target species
Bs-SecDF-F	ATG​AAA​AAA​GGA​CGC​TTG​ATT​GCG​TTT​TTC	1400	B. subtilis
Bs-R	CCC​GGC​AAC​CGT​GAC​CGC​ACT
Bs-SecDF-F	ATG​AAA​AAA​GGA​CGC​TTG​ATT​GCG​TTT​TTC	1140	B. sonorensis
Bso-R	TCT​GAC​AGA​GCT​CGT​ACC​AAA​GAT​GAA​C
Bs-SecDF-F	ATG​AAA​AAA​GGA​CGC​TTG​ATT​GCG​TTT​TTC	589	B. atrophaeus
Bat-R	CAT​ATT​TAG​GAT​TCT​CTT​TTT​GCA
Bs-SecDF-F	ATG​AAA​AAA​GGA​CGC​TTG​ATT​GCG​TTT​TTC	900	B. mojavensis,
Bmo-R	GTC​AAA​AAT​CTG​GAG​TGT​AAT​GTA​TAC​ATA​AAC​T
Bs-SecDF-F	ATG​AAA​AAA​GGA​CGC​TTG​ATT​GCG​TTT​TTC	1600	B. amyloliquefaciens
Ba-R	TTC ACG GAT CTT TTC TTT AGA TGG CTC TTCG
Bs-SecDF-F	ATG​AAA​AAA​GGA​CGC​TTG​ATT​GCG​TTT​TTC	840	B. licheniformis
BL-R	AGG​CAA​GCG​ATA​GTA​AAG​CAT​AAA​TAA
Bs-SecDF-F	ATG​AAA​AAA​GGA​CGC​TTG​ATT​GCG​TTT​TTC	450	B. aerophilus
Bae-R	GCT​TGA​TCG​TCA​CGA​TCG​GCT​CAT​TTG​TGT