Research Article
Involvement of the Bacillus SecYEG Pathway in Biosurfactant Production and Biofilm Formation
Table 2
Constructions, primers, and restriction enzymes used in this work.
| Name of primers | Primer sequences (5′----3′) | Restriction enzymes | Size of PCR fragment (bp) | Characteristics | Given name | References |
| SecAs5 | 5′AGGATCCCTTGGAATTTTAAATAAAATGTTTGATCC-3′ | BamHI | 2523 | pGEX6P-1-SecA | pKIKO3 | This study | SecAAs5 | 5′TGTCGACCTATTCAGTACGGCCGCAGCAAT-3′ | SalI |
| TasASF TasAasF | 5′TAGGATCCGGTATGAAAAAGAAATTGAG-3′ 5′TGTCGACTTAATTTTTATCCTCGCTATGCGCTTTTTC3′ | BamHI–SalI | 786 | pGEX4T1-TasA | pKIKO4 | This study |
| TapA-sF TapA-asF | 5′TAGGATCCTTTCGATTGTTTCACAATCAGC-3′ 5′TGTCGACTTACTGATCAGCTTCATTGCTTTTTTC-3′ | BamHI–SalI | 762 | pGEX4T1-TapA | pKIKO5 | This study |
| AprE1 AprE2 | 5′TCTCGAGGTGAGAAGCAAAAAATTGTGGATCAG-3′ 5′AGGTACCTTGTGCAGCTGCTTGTACGTTGAT-3′ | Xho1–KpnI | 1146 | pBAD-aprE-His | pKIKO9 | This study |
| AmyE-s AmyE-As | 5′TCTCGAGATGTTTGCAAAACGATTCAAAACCTC-3′ 5′AGGTACCATGGGGAAGAGAACCGCTTAAGCCCG-3′ | Xho1–KpnI | 659 | pBAD-amyE-His | pKIK10 | This study |
| TasA-TasA-As s | 5′TCTCGAGATGGGTATGAAAAAGAAATTGAG-3′ 5′AGGTACCATTTTTATCCTCGCTATGCGCTTTTTC-3′ | Xho1–KpnI | 786 | pBAD-TasA-His | pKIKO15 | This study |
| TapA-S TapA-aS | 5′TCTCGAGATGTTTCGATTGTTTCACAATCAGC-3′ 5′AGGTACCCTGATCAGCTTCATTGCTTTTTTC-3′ | Xho1–KpnI | 762 | pBAD-TapA-His | pKIKO20 | This study |
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