Epigenetic Methylation of Parathyroid CaR and VDR Promoters in Experimental Secondary Hyperparathyroidism

Figure 3

Methylation detection of CaR and VDR promoter regions in parathyroid glands. Analysis of the CaR and VDR promoter regions by PCR melting curve analysis after bisulfite treatment of genomic DNA. CaR is covered by PCR products CaR#1-2, and VDR is covered by PCR products VDR#1–4. Commercial low- and high-methylated rat DNA is analyzed in all of the PCR reactions shown in the “Methylation control” panel in green and red, respectively; it is also included in the “Sham,” “Uremia,” and “in vitro” panels. Control of methylation detection verified high levels of methylation in H19 and VDR downstream gene regions.