Morphological Retrospective Study of Peritoneal Biopsies from Patients with Encapsulating Peritoneal Sclerosis: Underestimated Role of Adipocytes as New Fibroblasts Lineage?

Table 1

Details of immunohistochemistry studies performed on peritoneal tissue biopsies (primary antibodies and corresponding cells specificity, retrieval processes, dilution, and type of secondary antibodies).

Antigen	Specificity	Retrieval; (time in minutes)	Dilution	Manufacturer
Secondary antibody: monoclonal
Podoplanin	Mesothelium, lymphatics endothelium	H1 (20)	1/50	Covance, Princeton, New Jersey, USA
Secondary antibody: polyclonal
AE1/AE3	Mesothelium	H1 (20)	1/800	Dako, Glostrup, Denmark
Calretinin	Mesothelium	H1 (20)	1/150	Menarini, Zaventem, Belgium
CD8	Cytotoxic/suppressor T cells subpopulation	H1 (20)	1/800	Dako, Glostrup, Denmark
CD20	B cells	H1 (30)	1/3000	Dako, Glostrup, Denmark
CD68	Monocytes/macrophages	H1 (30)	1/100	Menarini, Zaventem, Belgium
Secondary antibody: peroxidase
CD4	Helper T cells subpopulation	H2 (20)	1/50	Klinipath, Duiven, Netherlands
CD31	Endothelium	H2 (30)	1/1000	Menarini, Zaventem, Belgium
Vimentin	Mesenchymal cells	/	1/100	Klinipath, Duiven, Netherlands
α-SMA	Myofibroblasts	/	1/100	Menarini, Zaventem, Belgium

H1: citrate buffer, pH 6.0; H2: EDTA buffer, pH 8.0.