Aberrant Methylation Inactivates Transforming Growth Factor 𝛽 Receptor I in Head and Neck Squamous Cell Carcinoma

Table 2

Correlation between, tumor characteristics, protein and mRNA expression, and promoter methylation. Grading and tumor, lymph node, metastasis, and staging (TNM) are according to the 2002 UICC classification.

Tumor	Age	Site	Differentiation	Stage	Protein expression		▵ Expression	𝑇𝛽𝑅-𝐼 Methylation	𝑇𝛽𝑅-𝐼expression
					Normal	Carcinoma
1	82	HP	MD	IV	2+	1+	Decreased	NM
2	67	L	MD	IV	2+	0	Lost	NM
3	57	L	MD	IV	1+	0	Lost	M
4	64	L	PD	III	1+	0	Lost	NM
5	75	L	MD	II	1+	0	Lost	M
6	79	OC	MD	III	1+	0	Lost	NM
7	56	OP	MD	IV	2+	1+	Decreased	NM
8	53	OP	MD	IV	1+	0	Lost	M
9	71	OC	WD	III	1+	0	Lost	M
10	84	OP	MD	III	2+	0	Lost	M	2
11	73	L	MD	IV	1+	0	Lost	M	3
12	67	OC	WD	III	2+	0	Lost	M	3
13	38	L	MD	I	1+	0	Lost	M	2
14	48	HP	MD	1V	na	na	na	NM
15	62	OP	MD	II	2+	1+	Decreased	M
16	58	L	MD	III	3+	0	Lost	M	2
17	66	OC	PD	III	1+	0	Lost	NM
18	50	L	SCC	IV	2+	0	Lost	NM
19	55	OC	MD	IV	na	na	na	M	3
20	58	OC	WD	IV	2+	0	Lost	NM
21	46	OC	WD	III	3+	0	Lost	M
22	66	OC	MD	I	1+	0	Lost	M	3
23	70	L	WD	IV	2+	0	Lost	M	2
24	51	OP	WD	IV	1+	0	Lost	M
25	74	HP	MD	IV	na	na	na	NM	1
26	56	OC	MD	I	3+	0	Lost	M	2
27	66	HP	PD	III	1+	0	Lost	M	2
28	68	OC	MD	II	2+	0	Lost	M	2
29	81	OC	MD	IV	3+	0	Lost	M	3
30	55	L	MD	II	2+	0	Lost	M
31	44	L	MD	IV	1+	0	Lost	M	2
32	55	OC	MD	IV	1+	0	Lost	M	3
33	84	OC	SCC	I	na	na	na	NM	3
34	56	OC	WD	II	1+	1+	Unchanged	NM	1
35	74	HP	PD	IV	1+	0	Lost	M	2
36	60	OC	PD	II	3+	0	Lost	NM	1
37	60	L	WD	IV	3+	0	Lost	M
38	56	L	WD	II	na	na	na	M	3
39	56	L	MD	IV	3+	1+	Decreased	NM	1
40	48	L	MD	III	2+	0	Lost	NM	2
41	62	OC	MD	IV	1+	0	Lost	NM	2
42	65	L	WD	IV	3+	0	Lost	NM
43	47	OC	MD	III	3+	1+	Decreased	M	3
44	75	L	MD	III	na	na	na	M
45	51	OC	WD	IV	3+	3+	Unchanged	M
46	49	OC	WD	IV	na	na	na	M
47	70	OC	MD	IV	na	na	na	NM
48	47	OC	MD	III	1+	0	Lost	NM
49	50	HP	MD	IV	1+	0	Lost	M	2
50	72	OC	WD	IV	2+	0	Lost	M

OC = Oral cavity, OP = Oropharynx, HP = Hypopharynx, LA = Larynx, na = Not available. 1 = Fully expressed; 2 = Down-regulated; 3 = Not expressed. WD = Well differentiated, MD = Moderately differentiated, PD = Poorly differentiated, SCC = Squamous cell carcinoma