Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B
Figure 2
Construct for the transformation and herbicide resistance assay of putative transgenic plants. (a) T-DNA region of the binary vector plasmid pCAMBIA3301 harboring Arabidopsis PHYB gene. RB: right border; LB: left border; : CaMV 35S promoter; Pubi: ubiquitin promoter; intron GUS: GUS coding region with catalase intron insertion; PHYB: full-length phyB gene of Arabidopsis; BAR: phosphinotricin acetyltransferase gene coding region; T1: CaMV 35S gene terminator; T2: A. tumefaciens NOS gene terminator. Arrows indicate directions of transcription. (b) Herbicide resistance assay. Numbers in lanes represent putative transgenic plants selected for this analysis. 0.4% BASTA was sprayed onto nontransformed wild-type control plant (NT) and the transgenic plants, and the herbicide resistance of the plants was determined 14 days later.
