Research Article
Physicochemical Characterization of Thermally Treated Chitosans and Chitosans Obtained by Alkaline Deacetylation
Table 3
Conditions of alkaline deacetylation of chitin and purification of chitosan samples.
| | Batch | 1 | 2 |
| | Temperature (°C) | 100–110 | 110–122 |
| | Time (minutes) | 60 | 80 |
| | Cooling |
| | Centrifugation 10,000 g for 5 minutes |
| | Washing of pellet until low conductivity is achieved |
| |
Solubilization in 0.15 dm3/L lactic acid and orbital shaker at 200 opm |
| | Centrifugation 10,000 g for 5 minutes |
| | Obtention of supernatant |
|
Neutralization of supernatant with NaOH 1 dm3/L until
pH = 8.5 is reached |
| | Washing of suspension until low conductivity is achieved |
| | Freezing at −80°C and lyophilization |
| | Sample | D | E |
|
|
