Fish Collagen Promotes the Expression of Genes Related to Osteoblastic Activity

Table 2

Primers designed for RT-PCR analyses of gene related to calcification.

Gene name	Oligonucleotides
Alkaline phosphatase (ALP)	Forward: GTTGCCAAGCTGGGAAGAACAC
	Reverse: CCCACCCCGCTATTCCAAAC
Osteopontin (OPN)	Forward: CATTGCCTCCTCCCTCCCGGTG
	Reverse: GCTATCACCTCGGCCGTTGGGG
Osteocalcin (OCN)	Forward: AGGGAGGATCAAGTCCCG
	Reverse: GAACAGACTCCGGCGCTA
Bone sialoprotein (BSP)	Forward: TGTCTGCTGAAACCCTTC
	Reverse: GGGGTCTTTAAGTACCGGC
Ribosomal protein S17 (rpS17)	Forward: GCATATCATGCAACGCTTTC
	Reverse: GGAGCTTCAGCATCTCCTTG