Thermal Synthesis of Polypeptides from N-Butyloxycarbonyl Oligopeptides Containing Aspartyl Residue at C-Terminus

Figure 12

Chromatograms of the crude products obtained from Boc-Gly-Gly-L-Asp (a) at 120°C for 4 h and Boc-L-Val-L-Asp (b) at 130°C for 2 h in the heating reactions using an HPLC equipped with a TSK-GEL G-3000PW (300 mm × 7.5 mm i.d.). Eluent: 0.1 M sodium phosphate buffer, pH 6.9; flow rate: 0.7 mL/min; detection: UV 230 nm.

(a)

(b)