Hyperplastic Polyps Are Innocuous Lesions in Hereditary Nonpolyposis Colorectal Cancers
Figure 2
Pyrograms for MINT 1. (a) Represents unmethylated control bisulfite modified DNA. Position 2 represents the quality control loci and should remain negative for Cs. It can be seen that all Cs within the CpG islands at positions 1, 3, and 4 also reveal no Cs, that is, the DNA is unmethylated. In contrast, pyrogram B which represents a positive control from the SW48 cell line, position 2 remains negative for Cs, but the CpG’s at position 1, 3, and 4 have 64.1%, 87.5%, and 83.9% methylation, respectively. This positive control as anticipated is methylated. The final sample in C represents DNA extracted from a hyperplastic polyp. This demonstrates 20.6%, 27.3%, and 28.1% methylation at position 1, 3, and 4, respectively, with a negative result for the internal control at position 2.