Hyperplastic Polyps Are Innocuous Lesions in Hereditary Nonpolyposis Colorectal Cancers

Table 1

Primers used in the amplification of CpG islands from bisulfite-treated DNA by PCR (nested PCR) including their sequences and annealing temperature.

Gene	Genbank accession number	Sense primer	Antisense primer	Annealing temp (°C)
P16^INK4a	AF527803; NM_058195	GGTTTTTTTTAGAGG; ATTTGAGGGATA	AAACAAACCCTC; TACCCACCTAA	62
MGMT	AL355531	GGGTAATTTGGGAG; GTAT	CTCTCTTACTTTT; CTCAAATCCT	58
MLH-1	U83845	TTAGATTATTTTAGT; AGAGGTATATAAG	ATACCTTCAACC; AATCACCTCAATA	53