Research Article

Hyperplastic Polyps Are Innocuous Lesions in Hereditary Nonpolyposis Colorectal Cancers

Table 2

Primers used in the analysis of methylation at the amplified CpG islands of bisulfite treated DNA by PCR including their sequences and annealing temperature.

GeneMSP reactionSense primerAntisense primerAnnealing temp (°C)

UnmethylatedTTATTAGAGGG
TGGGGTGGATTGT
CAACCCCAAACC
ACAACCATAA
64
MethylatedTTATTAGAGGGTGG
GGCGGATCGC
GACCCCCGAACC
GCGACCGTAA
77.5
MGMTUnmethylatedTTTGTGTTTTGATGT
TTGTAGGTTTTTGT
AACTCCACACTCT
TCCAAAAACAAAACA
65
MethylatedTTTCGACGTTCGTAG
GTTTTCGC
TTTCGACGTTCGT
AGGTTTTCGC
70
MLH-1UnmethylatedTTAATAGGAAGAGT
GGATAGTG
TCTATAAATTACT
AAATCTCTTCA
57
MethylatedTTAATAGGAAGAGC
GATAGC
CTATAAATTACTA
AATCTCTTCG
60.5