Research Article

Hyperplastic Polyps Are Innocuous Lesions in Hereditary Nonpolyposis Colorectal Cancers

Table 4

PCR conditions for the methylated/unmethylated step.

StepTemperatureDuration

Initial denaturation95°C 4 min
Denaturation95°C30 sec × 35
Annealing(Primer specific)°C 30 sec × 35
Synthesis72°C 1 min × 35
Final extension72°C30 min
Holding4°C