Hyperplastic Polyps Are Innocuous Lesions in Hereditary Nonpolyposis Colorectal Cancers

Table 4

PCR conditions for the methylated/unmethylated step.

Step	Temperature	Duration
Initial denaturation	95°C	4 min
Denaturation	95°C	30 sec × 35
Annealing	(Primer specific)°C	30 sec × 35
Synthesis	72°C	1 min × 35
Final extension	72°C	30 min
Holding	4°C	—