Assessment of Nicotine Degradation in Cigarette Smoke under Different Storage Conditions (Light and Duration)

Table 1

Basic experimental information for this study.

	Experimental stage 1	Experimental stage 2
Sample type	Nicotine solution	3R4F reference cigarette
Sampling condition	Spiking^a	HCI regimen^b
Light condition	(1) Dark
	(2) Visible radiation; (i) Lamp: fluorescent lamp (Bioultra6, Telstar Technologies, Spain); (ii) Measuring device: light meter (LX-1108, Lutron Electronic Enterprise Co., Ltd., Taiwan); (iii) Irradiance:  W m^2 (about  Lux); (iv) Distance between light source and sample: 0.25 m
	(3) UV radiation (254 nm); (i) Lamp: UV lamp (TN-4LC, Korea Ace Sci., Republic of Korea); (ii) Measuring device: UVC light meter (UVC-254SD, Lutron Electronic Enterprise Co., Ltd., Taiwan); (iii) Irradiance:  W m^2; (iv) Distance between light source and sample: 0.25 m
Container temperature (°C)
Nicotine concentration (ng μL^−1)	40.4^c	80.4^c
Sampler	44 mm Cambridge filter
Exposure time (h)	0, 6, 12, 24, and 48	(1) Dark and visible: 48; (2) UV: 0, 6, 12, 24, and 48
Sample pretreatment	Methanol extraction (10 mL)

^a20 μL reagent-grade nicotine (purity, ≥99%) was spiked on the Cambridge filter. ^bPuff volume: 55 mL; puff interval: 30 s; puff duration: 2 s. ^cExperimental stage 1: theoretical concentration; experimental stage 2: mean analytical concentration.