Indoor Air

Research Article

Development of a Bioaerosol Sampling Method for Airborne Pathogen Detection with Focus on SARS-CoV-2

Table 5

Results obtained after PCR analysis only for the 5 (out of 21) field blanks collected at the cafeteria, schools, and daycare facilities which were not completely clean.


Infectious agent	Cafeteria blank 1	Cafeteria blank 2	Cafeteria blank 3	Daycare blank 1	School blank 1

Varicella-zoster virus DNA	Negative	Negative	Negative	Negative	Negative
Herpes simplex virus DNA	Negative	Negative	Negative	Negative	Negative
Bordetella pertussis DNA	Negative	Negative	Negative	Negative	Negative
Bordetella parapertussis DNA	Negative	Negative	Negative	Negative	Negative
Bordetella holmesii DNA	Negative	Negative	Negative	Negative	Negative
Chlamydophila pneumoniae DNA	Negative	Negative	Negative	Negative	Negative
Legionella pneumophila DNA	Negative	Negative	Negative	Negative	Negative
Mycoplasma pneumoniae DNA	Negative	Negative	Negative	Negative	Negative
Streptococcus pneumoniae DNA	Negative	Negative	Negative	Negative	Negative
Adenovirus DNA	Negative	Negative	Negative	Negative	Negative
Coronavirus 229E RNA	Negative	Negative	Negative	Negative	Negative
Coronavirus HKU1 RNA	Negative	Negative	Negative	Negative	Negative
Coronavirus NL63 RNA	Negative	Negative	Negative	Negative	Negative
Coronavirus OC43 RNA	Negative	Negative	Negative	Negative	Negative
SARS-CoV-2 RNA (RT-PCR)	LV ()	LV ()^†	LV ()^†	LV ()^†	Negative
Human metapneumovirus RNA	Negative	Negative	Negative	Negative	Negative
Bocavirus DNA	Negative	Negative	Negative	Negative	Negative
Bocavirus DNA	Negative	Negative	Negative	Negative	Negative
Rhinovirus	Negative	Negative	Negative	Negative	LV ()
Influenza A virus RNA	Negative	Negative	Negative	Negative	Negative
Influenza B virus RNA	Negative	Negative	Negative	Negative	Negative
Parainfluenza 1 virus RNA	Negative	Negative	Negative	Negative	Negative
Parainfluenza 2 virus RNA	Negative	Negative	Negative	Negative	Negative
Parainfluenza 3 virus RNA	Negative	Negative	Negative	Negative	Negative
Parainfluenza 4 virus RNA	Negative	Negative	Negative	Negative	Negative
Enterovirus RNA	Negative	Negative	Negative	Negative	Negative
RSV A RNA	Negative	Negative	Negative	Negative	Negative
RSV B RNA	Negative	Negative	Negative	Negative	Negative

Limit value = genetic marker was detected in the sample, but at a high Ct value (close to the detection limit). ^†Ct > LoB: for SARS-CoV-2, indicates samples with increased probability of being false positives.