Development of a Bioaerosol Sampling Method for Airborne Pathogen Detection with Focus on SARS-CoV-2

Table 6

Results of qPCR analysis from the air samples collected with the Coriolis μ at the two daycare facilities (Ct values shown are for SARS-CoV-2; the presence of other pathogens is indicated by superscript numbering).

			Week 1		Week 2
			Day 1	Day 2	Day 3	Day 4
C1	Playing room	M	LV (CT 36.55)^1	Negative	LV (CT 36.99)	Positive (, CT 33.86)^5
		A	—	Negative	LV (CT 34.66)	Negative
	Bedroom	A	Negative^2	—	LV (CT 35.33)^3	—
C2	Ground floor room	M	Negative	Negative	LV (CT 36.16)	LV (CT 39.82)^†
		A	—	Negative	Negative^4	Negative
	1^st floor room	M	Negative	Negative	Negative	LV (CT 36.82)^6
		A	—	Negative	—	Negative

LV = limit value. ^†Ct > LoB: increased possibility of false-pos; vl = viral load; M = sample collected in the morning; A = sample collected in the afternoon. ¹Streptococcus pneumoniae DNA positive (). ²Streptococcus pneumoniae DNA positive () + coronavirus OC43 RNA at LV (). ³Streptococcus pneumoniae DNA positive () + rhinovirus positive (). ⁴Influenza A virus RNA at LV ( CDC/38 Schülze). ⁵Streptococcus pneumoniae DNA positive () + rhinovirus positive () + influenza A virus RNA at LV ( CDC/41 Schülze) + enterovirus RNA at LV (). ⁶Rhinovirus positive ().