Cryptococcus neoformans as a Model for Radioimmunotherapy of Infections
Figure 5
Comparison of RIT and amphotericin efficacy towards nonmelanized and melanized CN in vitro and in vivo. (a–c) In vitro killing and dosimetry of melanized and nonmelanized CN cells treated with: (a) Re-labeled 8B7 and control isotype-matching MOPC21 mAbs; (b) Bi-labeled 18B7 and control MOPC21 mAbs; (c) amphotericin B; mel: melanized CN cells; non-mel: nonmelanized CN cells; (d, e) CFUs in the lungs and brains of mice infected with nonmelanized or melanized CN. AJ/Cr mice were infected IV with 3 × 105 CN cells and 24 hr later either given 100 μCi Bi-18B7 RIT or amphotericin B at 1 μg/g body weight on Days 1, 2, and 3 after infection or combined treatment or left untreated: (d) nonmelanized CN; (e) melanized CN. Detection limit of the method was 50 CFUs. No CFUs were found in the brains and lungs of mice infected with melanized CN cells and treated with RIT which are presented in the graph as 40 CFUs/organ; (f) CFUs in the brain and lungs of mice infected with 3 × 105 melanized (M) or nonmelanized (NM) CN cells and treated with amphotericin B at 1 μg/g body weight for 14 days. Mice were sacrificed at days 7 and 14 posttreatment; adapted from [26].
