Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates

Table 1

Oligonucleotide primers used for extended spectrum β-lactamase and virulence gene detection.

Gene name	Type	Primer Sequence	Annealing temp.	Amplicon size (bp)	Reference
SHV	Fw	5′-ACTATCGCCAGCAGGATC-3′	53°C	356	This study
	Rev	5′-ATCGTCCACCATCCACTG-3′
TEM	Fw	5′-GATCTCAACAGCGGTAAG-3′	50°C	786	This study
	Rev	5′-CAGTGAGGCACCTATCTC-3′
CTX-M-15	Fw	5′-GTGATACCACTTCACCTC-3′	49°C	255	This study
	Rev	5′-AGTAAGTGACCAGAATCAG-3′
TraT	Fw	5′-GGTGTGGTGCGATGAGCACAG-3′	63°C	290	[23]
	Rev	5′-CACGGTTCAGCCATCCCTGAG-3′
FimH	Fw	5′-TACTGCTGATGGGCTGGTC-3′	50°C	640	[24]
	Rev	5′-GCCGGAGAGGTAATACCCC-3′
Iss	Fw	5′-GGCAATGCTTATTACAGGATGTGC-3′	50°C	260	[24]
	Rev	5′-GAGCAATATACCCGGGCTTCC-3′
BssS	Fw	5′-GATTCAATTTTGGCGATTCCTGC-3′	48°C	225	[24]
	Rev	5′-TAATGAAGTCATTCAGACTCATCC-3′
iucA	Fw	5′-CGAAATCGAAATAGATCACC-3′	51°C	1125	[24]
	Rev	5′-CTGACGCGATTTGCCGC-3′

Forward, reverse, and base pair.