The Pathogenic Aspects of Human Parvovirus B19 NS1 Protein in Chronic and Inflammatory Diseases

Table 2

Thirteen cytokines’ concentration of mock-transfected, B19 NS1-transfected, and untransfected HEK-293T cell are measured at 24, 48, and 72 hr posttransfection. Cytokine concentrations are reported based on a picogram per milliliter as mean. The experiments were carried out two times in duplicate.

Posttransfection time (h)

HEK-293T

Cytokines (pg/ml) mean ± SD

IL-13

IL-10

IL-4

IL-2

IL-6

IL-9

IFN-γ

TNF-α

IL-17A

IL-17F

IL-21

IL-22

IL-5

24

Untransfected cell

1.21 ± 0.19

1.67

1.37

1.25 ± 1.18

1.15 ± 0.68

1.4 ± 0.43

0.95 ± 0.02

0.88 ± 0.02

3.42 ± 0.2

1.24

2.62 ± 0.16

43.8 ± 0.3

0.64

Mock-transfected

1.21 ± 0.19

1.67

1.37

1.25 ± 1.18

2.61 ± 0.09

1.64 ± 0.1

1.02 ± 0.02

5.98 ± 0.5

7.88 ± 1.0

1.24

6.86 ± 1.7

63.6 ± 0.9

0.64

NS1- transfected

1.72 ± 0.35

1.67

1.37

4.37 ± 0.19

8.56 ± 3.56

2.6 ± 1.44

1.02 ± 0.02

7.44 ± 0.7

16.47 ± 1.5

1.24

8.58 ± 2

72.48 ± 3.0

0.64

48

Untransfected cell

1.21 ± 0.13

1.67

1.37

1.25 ± 1.12

2.26 ± 0.78

1.26 ± 0.28

0.95 ± 0.02

5.81 ± 0.5

3.90 ± 0.24

1.24

1.98 ± 0.2

30.42 ± 0.4

0.64

Mock- transfected

1.21 ± 0.13

1.67

1.37

1.25 ± 1.12

3.99 ± 1.66

1.68 ± 0.19

0.95 ± 0.02

6.15 ± 0.3

4.25 ± 0.1

1.24

3.17 ± 1.4

61.01 ± 0.7

0.64

NS1- transfected

1.67 ± 0.14

1.67

1.37

1.99 ± 0.35

11.23 ± 0.71

2.46 ± 1.9

0.95 ± 0.02

6.32 ± 1.0

18.8 ± 1.1

1.24

12.58 ± 0.18

62.88 ± 1.2

0.64

72

Untransfected cell

1.52 ± 0.32

1.67

1.37

1.25 ± 0.16

3.12 ± 0.42

1.7 ± 0.14

1.09 ± 0.09

6.01 ± 0.4

10.48 ± 1

1.24

10.15 ± 1.2

53.30 ± 2.1

0.64

Mock- transfected

1.67±

1.67

1.37

2.62 ± 0.58

8.47 ± 1.73

2.12 ± 0.1

1.32 ± 0.1

7.69 ± 0.6

14.29 ± 1.5

1.24

12.58 ± 0.5

56.71 ± 1.3

0.64

NS1- transfected

5.25 ± 2.15

1.67

1.37

13.50 ± 1.13

14.46 ± 2.09

4.65 ± 1.2

4.94 ± 0.8

23.49 ± 0.3

39.56 ± 1.2

1.24

17.71 ± 0.24

90.23 ± 3

0.64