Cloning, Expression Analysis, and Detection of the Vitellogenin in the Chinese Black Sleeper Bostrychus sinensis

Table 3

Organization dehydration program.

Reagents	Time
50% ethanol	20 min
70% ethanol	30 min
80% ethanol	30 min
95% ethanol	1.0 h
100% ethanol I	15 min
100% ethanol II	10 min
100% ethanol + 1/2 xylene	5 min
Xylene I	10 min
Xylene II	10 min
1/2 xylene + 1/2 paraffin	1.0 h
Paraffin I	1.5 h
Paraffin II	1.5 h
Paraffin III	2.0 h