Research Article

Cloning, Expression Analysis, and Detection of the Vitellogenin in the Chinese Black Sleeper Bostrychus sinensis

Table 4

Water dewaxing program.

ReagentsTime (min)

Dimethylbenzene III5
Dimethylbenzene II5
Dimethylbenzene I2
1/2 dimethylbenzene + 1/2 ethanol2
100% ethanol III2
100% ethanol II2
100% ethanol I2
95% ethanol2
85% ethanol1
70% ethanol1
50% ethanol1
50% ethanol1