Research Article
Cloning, Expression Analysis, and Detection of the Vitellogenin in the Chinese Black Sleeper Bostrychus sinensis
| Reagents | Time (min) |
| Dimethylbenzene III | 5 | Dimethylbenzene II | 5 | Dimethylbenzene I | 2 | 1/2 dimethylbenzene + 1/2 ethanol | 2 | 100% ethanol III | 2 | 100% ethanol II | 2 | 100% ethanol I | 2 | 95% ethanol | 2 | 85% ethanol | 1 | 70% ethanol | 1 | 50% ethanol | 1 | 50% ethanol | 1 |
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