Cloning, Expression Analysis, and Detection of the Vitellogenin in the Chinese Black Sleeper Bostrychus sinensis

Table 5

HE staining program.

Reagents	Effects	Time
Hematite dyeing solution	Dyeing	5 min
0.5% chemicalbookcheck	Color separation	2 s
1% aqueous ammonia	Return to blue	1 min
1% eosin	Dyeing	15 s
80% ethanol	Rinse	15 s
95% ethanol	Rinse	15 s