Cloning, Expression Analysis, and Detection of the Vitellogenin in the Chinese Black Sleeper Bostrychus sinensis

Table 6

List of qRT-PCR program.

Stage	Temperature (°C)	Time
Predegeneration	95	3 min
40 times cycle	95	10 s
	60	10 s
	72	10 s
The third stage	95	30 s
	60	5 s
	95	5 s