Establishment of SYBR Green I Real-Time PCR for Detection of Streptococcus agalactiae in Aquaculture Waters

Figure 1

Gene cfb amplification and construction of recombinant plasmid. (a) Amplification and detection of complete cfb gene. M, Marker DL5000; 1, negative control without template; 2, complete cfb gene was amplified by cfb F/R primer with the template of total S. agalactiae DNA genome. (b) Validation of cfb fluorescent quantitative primer cfb-qF/R. M, Marker DL5000; 1, negative control without template; 2, partial fragment cfb gene was amplified by cfb-qF/R with the template of the reconstructed plasmid pMD18-T-cfb.

(a)

(b)