Research Article
Establishment of SYBR Green I Real-Time PCR for Detection of Streptococcus agalactiae in Aquaculture Waters
Figure 2
Amplification curve of real-time PCR using SYBR Green I assay and the standard curve. (a) Amplification curve of real-time PCR for detection of tenfold serial dilutions of recombinant plasmid pMD18-T-cfb after 40 cycles. Curves 1–8: 1.57 × 109, 1.57 × 108, 1.57 × 107, 1.57 × 106, 1.57 × 105, 1.57 × 104, 1.57 × 103, and 1.57 × 102 copies/μL pMD18-T-cfb, respectively. (b) Standard curve generated from the mean of Ct values against log10 of tenfold serial dilutions of recombinant plasmid pMD18-T-cfb.
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